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At baseline, trained nurse interviewers administered a structured questionnaire to each subject to collect comprehensive information on demographics, occupation, lifestyle habits, medical history, usage of medication and dietary supplements, alcohol consumption, and past cigarette smoking history

Although surface proteins such as CD44 and CD133 have been widely used to isolate CSC populations, these markers are not exclusive to CSCs and are often expressed in normal stem cells (NSCs) or non-tumorigenic cancer cells

In addition, for each biological replicate, 3 technical replicates were performed

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In: Operative Standards for Cancer Surgery:Thyroid, Gastric, Rectum, Esophagus, Melanoma