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Briefly, a standard 25 l reaction mixture contained in final concentration of 1 KAPA PROBE FAST qPCR master mix buffer, 0.4 M of each forward and reverse primers, 0.2 M Probe for H-FABP, CPT IB, ACC2, MCD and GAPDH (Table 1), 100 ng of cDNA and RNase, DNase free water

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