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Drug solutions, typically 5 mg/ml in HPLC-grade water (except for Dihexa, prepared in DMSO), were added to rat serum, and the mixture was incubated at 37C
as a result, surfactant production decreases markedly, which in turn causes alveolar fluid accumulation leading to alveolar collapse and ARDS (Matthay and Zemans, 2011
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