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After transfection for 24 hours, IL-13 was added into lower chamber media every 48 hours for up to 7 days

So, unlike topical self-tanners that tint the outer layer of your skin and dont have any internal, systemic effects, melanotan is acting within the cells of the body just like UV radiation

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Booth, S.L., Golly, I., Sacheck, J.M., Roubenoff, R., Dallal, G.E., Hamada, K

The protein bands were detected using enhanced chemiluminescence (ECL) reagent (Pierce ECL Western Blotting Substrate, Thermo Scientific) according to the manufacturers instructions

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