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Use of balanced salt and culture media Working solutions and gradient solutions may also be prepared by diluting OptiPrep directly with any cell suspension media such as Hanks Balanced Salt Solution (HBSS) or serum-free culture medium (e.g

The expression of target genes was analysed via quantitative real-time PCR with a QuantaStudio 5 qPCR machine (Applied Biosystems, Thermo Fisher Scientific)

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