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After treatment, to assess mitochondrial membrane potential ( m ), A375 cells were incubated with 4 M JC-1 (ThermoFisher Scientific) in phenol red free DMEM for 20 min in a 5% CO 2 incubator at 37 C, then washed twice with DMEM without phenol red

Once reconstituted, refrigerate at 2-8C

For oral BPC-157 , it's best to take it on an empty stomach , ideally 3060 minutes before meals

Some individuals take oral supplements like N-acetylcysteine (NAC) or milk thistle to support glutathione production in between IV sessions

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