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We need to get it from our diet, which is where things can get tricky

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Originally developed as an anti obesity compound, it has become a widely used research tool for studying fat metabolism, lipolysis, and more recently cartilage repair, valued particularly for its ability to mimic the fatmetabolizing activity of growth hormone without the broader hormonal side effects that come with it

however, the only statistically significant difference was between the LD control group and both groups switched from WD to LD, regardless of their treatment (Supplementary Fig

Membrane pellets were homogenized and solubilized for 45 min in buffer containing 150 mM NaCl, 20 mM Tris (pH8.0), 29.5 mM n-dodecyl--D-maltopyranoside, and 6 mM cholesterol hemisuccinate at 4 C

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