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1 excitation: field of view, 24.0 cm, slice thickness, 1.4 mm) on the GE Signa scanner at the University of Fukui Hospital, and (echo time/repetition time, 4.4/12 ms

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Subsequently, the proteins were transferred onto polyvinylidene difluoride (PVDF) membranes and blocked for 90 min at room temperature of 20C25C using a 5% non-fat milk solution in tris-buffered saline tween-20

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Kuhn et al., 1996a