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Deacon CF, Nauck MA, ToftNielsen M, Pridal L, Willms B, Holst JJ

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Protein bioactive complexes promote osteogenesis under microgravity environment

Following incubation, caspase 3 activity was measured using the fluorogenic substrate AC-DEVD-AMC as described before ( 2 , 1.2 mM EGTA, 1.5% Nonidet P40, 0.3% CHAPS, 30% sucrose, 150 M caspase 3 substrate DEVD-AMC, 30 mM DTT, and 3.0 mM PMSF was directly added to HepG2 cells