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During the oxidation phase, accumulated ROS (such as hydrogen peroxide HO and superoxide anion O) in the cell can directly react with GSH in a non-enzymatic manner, or, under the catalysis of glutathione peroxidase (GPX), promote the oxidative coupling of two GSH molecules to form one molecule of GSSG, while releasing two protons (H) and two electrons (e)

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6,7 Flupirtine is metabolized in the liver by peroxidase enzymes to the active N-acetylated analogue D13223 and 4-fluorohippuric, which are further oxidised and then conjugated to inactive metabolites

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