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They were placed into a stereotaxic frame and their head was ventroflexed to enable access to the lower brainstem through blunt dissection of the neck muscles as described previously 7

Biocompatibility evaluation The cytocompatibility of the hydrogels was evaluated by Live-Dead staining and MTT assay

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The ATP-grasp fold is conserved within the ATP-grasp superfamily and is characterized by two alpha helices and beta sheets that hold onto the ATP molecule between them