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The protein sample was extracted from membrane by adding a 2 solubilization buffer containing 20 mM HEPES pH 7.5, 500 mM NaCl, 2% (w/v) n-dodecyl-beta-D-maltopyranoside (DDM, Affymetrix), 0.4% (w/v) cholesteryl hemisuccinate (CHS, Sigma), and 2% (w/v) glycerol for 3 h at 4 C

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Its a critical, non-negotiable step that transforms a stable, lyophilized powder into a usable solution, and getting it wrong can invalidate everything that follows

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The rationale underlying such a device is to ensure that nutrients are prevented from coming in contact with the absorptive surfaces of the proximal small bowel (76)