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Adult male MIA mice were paired with estrous female C57BL/6J mice in a clean cage placed inside a noise-limiting chamber equipped with an ultrasonic microphone

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Typically, a million cells were lysed in 1 ml of lysis buffer

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(21) Baseline measurements of P1NP, BMD, and CTX-1 were recorded, and participants were randomized into the semaglutide group to receive 1.0 mg or the placebo group