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Mol Cancer Ther 2007

The blots were incubated with primary antibodies against PGC-1 (Chemicon International, Temecula, CA) and AMP-activated protein kinase (AMPK) (Cell Signaling Technology, Beverly, MA), and the reaction products were visualized using a horseradish peroxidase-conjugated secondary antibody (Invitrogen, Carlsbad, CA) and enhanced chemiluminescence (Chemi-Lumi One Super, Nakarai Tesque, Kyoto, Japan) (Additional file 1: Figure S1)

In this way, the GST-GSH interaction could act as an anchor, holding the therapeutic protein on a hydrogel until MMP-2 cleaves the therapeutic protein from the GST anchor

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