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When this process falters, oxidative stress rises, damaging enzymes and cell membranes

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For instance, protein targets change when your appetite drops

Effects on maximum lifespan were determined by setting the threshold for lifespan to the 90th percentile for both groups combined 53

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After incubation, mitochondria were pelleted (1 min at 16,000 g ) and pellets were resuspended in 200 L of 50 mM HEPES buffer (pH 7.4) supplemented with 10 mM IAM-TPP, disrupted using mechanical micro-homogeniser and sonicated for 5 min