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MPO efficiency assessment When the cell density reached 80%, the cells were treated with three different conditions: transfection with the MPO plasmid and the addition of L-carnitine (3 mM, HY-B0399, MCE) and betaine (2 mM, HY-B0710, MCE)

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There were also downregulations observed in subunits of oxidative phosphorylation enzymes, but these were counterbalanced by concurrent significant upregulations in other subunits

Mass spectrometry confirmation Mass spectrometry confirms molecular identity by measuring the mass-to-charge ratio of the compound

Unreconstituted PT-141 vials should be stored refrigerated at 28C in lyophilized (powder) form, protected from light and moisture