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The untargeted analysis was carried with a data independent acquisition (DIA) mode in which the precursors were fragmented using HCD on a 350875 m/z precursor mass range using 35 DIA windows of 15 m/z with a 0.1 m/z overlap

If any of these mistakes happen and the solution looks cloudy, contains particles, or feels off, discard the vial and start fresh

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