The values obtained in the presence of KA and HQ were calculated from LineweaverBurk plots (Figs 1 and 2
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Rather than applying standardized dosing schedules universally, the program emphasizes individualized titration aligned with tolerance and metabolic response
The NHS and NICE recommend PDE5 inhibitors (sildenafil, tadalafil, vardenafil, avanafil) as first-line treatment, alongside lifestyle modifications and management of underlying conditions
In brief, 3,000 cells were seeded in a 96-well Seahorse culture plate, with six wells included per biological replicate