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Hackel et al., 2023)

Methods Cell culture The mouse triple-negative breast cancer cells, 4T1, were obtained from American Type Culture Collection (ATCC) and cultured in RPMI-1640 medium (Biowest, France) supplemented with L-glutamine (Biowest), 10% fetal bovine serum (FBS, Biowest), and 1% penicillin/streptomycin (Biowest) at 5% CO 2 at 37 C

Overweight and obesity are fundamentally consequences of excessive caloric intake, but the ever-increasing number of overweight people, despite its well-documented negative health effects, shows that restoring this imbalance is not easy, especially in our time of food abundance

5.03g of Fmoc-Pro-OH, 1.13g of HOBt and 1.2mL of DIC are weighed, DMF20mL is added for dissolution, and activation is carried out in ice bath for 10 min

112(1): 308 Pande SV

These studies describe the behavior of collagen produced by the tumor itself, not the effects of oral collagen supplements