All individuals were anesthetized with medetomidine (Domitor, Nippon Zenyaku Kogyo Co., Ltd, Fukushima, Japan) [0.05 mg/kg intramuscularly (IM)] and ketamine hydrochloride (Ketalar, Daiichi Sankyo Co., LTD, Tokyo, Japan) (10 mg/kg IM), and then veterinarians pressed their bladders gently to collect their urine samples
It is particularly important during pregnancy for foetal neural tube development
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