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Plant cell wall as a key player during resistant and susceptible plant-virus interactions

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The mRNA levels for modulated genes were determined by reverse transcription of total RNA followed by quantitative real-time PCR analysis (qRT-PCR) on a Quant Studio 6 Flex system (Applied Biosystems, Life Technologies, U.S.) using SYBR PCR Master Mix reagent kits (Takara, Japan) following the manufacturers protocol