Correction of I 405 for autofluorescence bleeding into the 405 nm channel and subtraction of background signals for each channel was performed
The presence of a single defined binding site for the conserved part of the R-domain confirms an anticipated but elusive binding site and may provide implications for several other types of transporters where such long phosphorylated unstructured domains or loops are widespread
If you're working with 100mcg, the 4mL dilution makes it a simple 4-unit draw, which is far more accurate than trying to estimate 1 unit on the 1mL dilution
Haruyama, J., Sodeyama, K., Han, L
Wen S, Zhang T, Tan T (2004) Utilization of amino acids to enhance glutathione production in Saccharomyces cerevisiae
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