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The elevation of these antioxidant compounds suggests that both substances may help protect against oxidative stress in the brain, though glutathione showed some interesting age-dependent effects that warrant further investigation

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After incubation with trypsin, the peptide concentration in samples was estimated using a Micro BCA Protein Assay (Thermo Scientific, #23235), and a volume corresponding to 25 g peptides was transferred to a new low-bind Eppendorf tube per sample

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