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In particular, the PI3K/AKT axis converts phosphatidylinositol-4,5-bisphosphate (PIP 2 ) to phosphatidylinositol 3,4,5-trisphosphate (PIP 3 )

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Before analysis, cells were washed once with PBS, incubated in 175 l XF base media (Agilent Technologies) supplemented with 2 mM glutamine with or without 100 nM insulin, and incubated at 37C without CO 2 for 1 hour

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