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Drug Target Identification and Validation The GST pull-down assay plays a significant role in drug discovery and development, particularly in identifying and validating drug targets

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Western blot analysis RIPA Lysis Buffer (TransGen, Beijing, China) was used to extract total protein from tissues/fibroblasts and a Nuclear and Cytoplasmic Protein Extraction Kit (TransGen) was used to extract the protein from nucleus and cytoplasm of fibroblasts

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published a comprehensive review on the use of GLP-1 RAs in plastic surgery, focusing for the first time primarily on their potential benefits and practical considerations in reconstructive procedures [96]