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For perfusion assays with HeLa cells, NAPstar3b was monitored using the Ex and Em settings as above, HyPer7 was monitored using- Ex: 405 20 nm, Em: 525 50 nm and Ex: 470 40 nm, Em: 525 50 nm

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Due to the lack of precise data on the effectiveness and safety of this method, it should be approached carefully, and medical consultation is recommended before use