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Results Control of TNF--dependent gene expression changes by GSTM1 in cultured astrocytes To evaluate the role of GSTM1 in astrocyte activation during acute brain inflammation, we examined the role of GSTM1 in TNF--dependent transcriptional program in primary mouse astrocytes

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Furthermore, we performed immunohistochemical staining of alkaline phosphatase (ALP) and ELISA tests of ALP and Type I collagen (COL I) expression in different groups

Both pathways culminate in the activation of caspases 3 and 7, which promote DNA fragmentation and loss of viability, respectively, leading to apoptosis