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6 and 7)

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As de novo synthesis of ceramide is located in the ER, which is absent in erythrocytes, it is thus assumed that erythrocytic ceramide should directly originate from the hydrolysis of sphingomyelin

It is always wise to consult with your healthcare provider before starting any new routine, especially if you are pregnant, nursing, or taking medication.

with 100 g TNP-Ficoll FITC (Biosearch Technologies F-1300F) and 30 min later tissues were analyzed with flow cytometry (splenocytes were stained with surface markers for the detection of FoB and MZB, with the addition of biotinylated anti-TNP (BD Pharmingen 554055) + Streptavidin-APC (Biolegend 405207 for TNP detection) or snap-frozen for immunofluorescence microscopy in OCT (Sakura 4583)