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TM3 cells were harvested after treatment, stained with Annexin V-FITC and PI reagent, and incubated in the dark for 15 minutes

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Inducers, such as erastin, induce ferroptosis by inhibiting cysteine uptake by system x c ( Osteoporosis Osteoporosis is characterized by an imbalance between bone resorption and bone formation, leading to a progressive loss of both trabecular and cortical bone mass and density

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