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Observation of Reactions: Monitor for any adverse effects and discuss with your provider promptly

For immunofluorescence (IF) staining, after deparaffinization and antigen retrieval, sections were incubated overnight at 4 C with a mouse anti-insulin antibody (1:200, GB15334, Servicebio), rabbit anti-PPAR antibody (1:50, ab23673, Abcam), or rabbit anti-UCP2 (1:200, DF8626, Affinity) antibody, followed by incubation with appropriate secondary antibodies (1:1000, 7074 S and 7076 S, CST)

Magnesium supplementation (200400 mg daily) is generally safe but consult your provider before adding supplements

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Combined deletion of Mfn1/2 in -cells reduces mtDNA content, impairs mitochondrial morphology and network, and compromises respiratory function, eventually resulting in severe glucose intolerance (Sidarala et al., 2022)