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Cell R software (Olympus Soft imaging solutions, Munster, Germany) or WinFluor Fluorescence Image Capture and Analysis program (John Dempster, University of Strathclyde, Scotland) was used to record excitation changes in intracellular calcium (OMalley et al., Figure 2 Figure 3 As previously described (OMalley et al., 2+ ] i ) that was greater than two standard deviations from the baseline values in that cell (calculated during the 150 s preceding stimulus application)

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