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A protocol that needs to compare the combination against each peptide alone (the only honest way to claim synergy) needs the singles in any case

8c and Supplementary Fig

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In this assay, the 30 min incubation duration was chosen to be optimal because of the inherently cytotoxic nature of CuCl 2 and Cu(OAc) 2 towards HaCaT keratinocytes, as they were observed to cause substantial cell death at a duration longer than 30 mins