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Confirm with a serum B12 test (below 300 pg/mL warrants supplementation

Moreover, the cell death is critically dependent specifically on mitochondrial protein adduct formation [27, 28] and scavenging of mitochondrial reactive oxygen species (ROS) and peroxynitrite after the protein binding occurs effectively protects against cell death [2932]

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In this context, we would like to stress an exceeding Glu/Gln ratio compared to control animals at prolonged 72 h reperfusion time in IPC animals, which suggests accelerated glutamate metabolism recovery