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Semaglutide was originally developed by Novo Nordisk as an antidiabetic agent under the brand name Ozempic (0.52 mg weekly, subcutaneous), approved by the FDA in 2017 for type 2 diabetes management

Currently, several senolytics were reported to kill senescent cells ( In this study, we tested the hypothesis that FOXO4-DRI treatment can selectively remove senescent cells in expanded human chondrocytes, thus enhancing their potential to generate high-quality cartilage

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M., Concepcion, B

doi: 10.1038/nrc3258 65 NguyenAHoLHoggRChenLWalshSRWanY

Transfection was carried out using Lipofectamine 3000 Kit on cells at 50% confluence for 6 hours, followed by protein extraction 48 hours post-transfection