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Shaking can denature the peptide, which means breaking apart its delicate amino acid structure
Molecules 27 , 7289 (2022)
Use proper injection technique and clean with alcohol wipes
Slow injection: Push the plunger slowly and pause a few seconds before withdrawing the needle to prevent backflow
All these processes which take place in the cell would not have been possible had the protein existed in a single structure or was flipping between only two states, active and inactive
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