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Taking it before fasted cardio is a common and effective protocol

Trimethylamine-N-oxide formation, the bacterial taxa involved and intervention strategies to reduce its concentration in the human body

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(2023) 83:186202.e11

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Depending on the needs of the study, prepared solutions may be maintained under refrigerated conditions for short-term use or frozen for longer-term laboratory storage, with aliquoting commonly used to help maintain sample integrity during repeated use