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The 2,2-dimethyl-tetrahydropyran moiety inserts between TM2 and TM3, forming hydrophobic contacts with aliphatic and aromatic residues lining this inter-helical space

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In CST-2,4-methylene-cholesterol complex, TYR176 (-2.14 kcal/mol) was found to be the dominant energy decomposed residue while the residues PHE177 (-1.36 kcal/mol) showed relatively lesser binding affinity

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