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p53 TAD (13-61) was subcloned into the pET His 6 glutathione-S-transferase (GST) TEV LIC cloning vector (2G-T, obtained from Scotta Gradia, Addgene plasmid #29707) and transformed into BL21 (DE3) pLysS cells

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Like any medication, it does have potential side effects, the most common being gastrointestinal (GI) issues Long-term studies are ongoing, but the benefits of significant weight loss, such as reduced cardiovascular risk and improved metabolic health, often outweigh these risks for many patients, says Dr

Greibe et al