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The exposure to environmental factors such as smoking, ultraviolet radiation (UV), heavy metals, ozone, allergens, drugs, toxins, and environmental pollutants can elevate ROS levels within cells
However, results develop progressively as the body adjusts to improved internal signalling
Protein lysates were boiled at 95 C for 10 min on a thermomixer (Thermo Fisher Scientific) and sonicated on the Bioruptor (Diagenode) system
1C), indicating dose-dependent excitatory effects