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856954, May 2022, doi: 10.3389/fendo.2022.856954

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the pool containing Arg 34 GLP-1 (7-37) and various impurities was diluted with one volume of water and two volumes of ethanol, and 20 ml of the resulting solution was applied to a 20 ml Source 15Q (Amersham Pharmacia Biotech) column equilibrated with 100 ml 20 mmol/kg Tris-hydroxymethyl amino-methane, 45 % (w/w) ethanol, pH 8.5