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DNA was not isolated from the first two samples collected in the LD/LD-V group, and the first sample collected in each of the other respective groups, due to the use of saline to flush the cecum (after weighing) during early collection (therefore, n = 25 cecal samples)

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This selective effect is crucial as it suggests that 5-amino-1MQ might target cancer cells more specifically without broadly affecting other cells [10]

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This timing allows AMPK activation to proceed without NAD+ limitation, maximising mitochondrial biogenesis and fat oxidation