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The rationale behind this strategy is that pyridostigmine is a reversible carbamate inhibitor of acetylcholinesterase, which at doses used to treat myasthenia gravis, will occupy and protect a substantial percentage of acetylcholinesterase from being able to bind irreversibly with soman

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PMID: 34167568 J Anim Sci 2022 Apr 1;100(4):skac069

Neither is inherently better. Using 2mL of water creates a more dilute solution, which can make it physically easier to measure out precise microgram doses, as youll be drawing a larger volume into the syringe

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