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Briefly, approximately 1.510 5 host cells (A549, RAW 264.7, PBL-985, and PBL-985 nox2 -KO cell lines) were seeded per well in 12-well plates and cultured in DMEM + 5% FBS at 37C and 5% CO2 for 24 h until reaching 95% confluence, equivalent to approximately 3 10 5 cells

[DOI] [PMC free article] [PubMed] [Google Scholar] 171.Villarreal-Barajas T., Vzquez-Durn A., Mndez-Albores A

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Because I think one thing that both clinicians and patients like to be guided by is, Okay, if Im not seeing at least some response by X time interval, then I should move on or tweak or modify. So is there a certain time of response that you find is typical