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Discarding the upper plasma, wash with three times volume of PBS for 3 times (re-suspend blood cell with PBS, centrifuge at 600 g for 10 minutes), add equal volume of Reagent I

429,430,431,432,433 The mechanism of enhanced glycolysis involves the activation of pyruvate kinase isoenzyme M2 (PKM2), which triggers downstream inflammatory factors in a hypoxia-inducible factor 1 (HIF-1)-dependent manner

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[20] Cytochrome isoforms affected by grapefruit components include CYP1A2, CYP2C9, and CYP2D6, [21] [22] [23] [24] [25] but CYP3A4 is the major CYP enzyme in the intestine