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After 12 h of T-cell costimulation with anti-CD3/CD28 antibodies in the absence of SFN, CD25 and CD69 were strongly expressed, while their expression was diminished in a dose-dependent manner in the presence of SFN (Figure 3A)

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To confirm the efficacy of hM4Di-mediated inhibition and hM3Dq-mediated excitation, CNO (10 M) was bath-applied

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