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The release of cytochrome C from the mitochondria leads to production of a caspase-activating complex of cytochrome c, APAF-1, and procaspase-9, triggers the up-regulation of caspase-9, and subsequently increases the expression of caspase-3, followed by the induction of apoptotic events in the cell [44]

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[DOI] [PubMed] [Google Scholar] 181.Diksic M., Young S.N

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Use the volume the calculator returns above and add that amount of bacteriostatic water to your one blend vial