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10.1016/j.parkreldis.2016.08.019 447 VagnerovK.VodikaM.HermanovP.ErgangP.rutkovD.KlusonovP.et al

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Briefly, the cells were washed with phosphate-buffered saline (PBS) once, centrifuged to remove residual medium, and gently resuspended in 195 L of binding solution

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The trade-off is an extra reconstitution step before the peptide can be worked with