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Peaks were manually validated based on signal intensity, transition chromatogram superposition and retention time

Participants were treated in a Florida health network between 2014 and 2024

Third, we chose to use overall survival as the primary endpoint, which is a hard outcome, providing a comprehensive assessment of the treatment effect in a retrospective study, although the proportion of patients becoming non-self-sufficient and ALSFRS-R total score, used as secondary outcomes, are validated endpoints for trials in ALS

doi: 10.1126/science.271.5257.1854

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