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Harmonic restraints of 50.0 kcal mol 1 2 were applied to the solutes, including the crystallographic ions, to allow solvent relaxation while preserving the maximum fidelity to the docking pose, and the Andersen temperature coupling scheme 81,82 was used to control and equalize the temperature

Although small-scale studies and disparities in technical proceduressuch as sample processing, sequencing, and bioinformatic analyseslimit the reliability and generalizability of the findings