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After 4 days, the medium was replaced with DMEM/F12 containing 3.3 mM biotin,1.7 mM pantothenic acid, 0.01 mg/ml transferrin, 20 nM insulin, 100 nM cortisol, 0.2 nM tri-iodothyronine every other day (Sigma-Aldrich)

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Henein MY, Vancheri S, Longo G, Vancheri F

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Revisiting the Ratio of Bacterial to Host Cells in Humans